Research Supervision Capacity
About Me
I have good knowledge of Microbiology & Immunology, especially skin transplantation, cancer Immunology, autoimmune diseases, PLGA nanoparticles generation, Flow cytometry, Cell Culture, Vaccinology, and Molecular Biology. I have competence with modern molecular biology techniques like different types of PCR, sequencing, Plasmid construction, protein extraction, purification, and SDS-PAGE. I have significant general computer experience. Using many advanced Molecular Biology/ Bioinformatics software.
Research Interest Keywords
Contact Info
Research Publication
1. Anam, M. Lashari, M.H. Akhtar, M.S. Abbas, M. Shahzad, K.A. Rubab, A. Kanwal, M. Tahreem, S. Alzahrani, K.J. Alsharif, K.F. Alzahrani, F.M. 2026. “The effects of hCG or GnRH treatment on embryonic mortality and reproductive performance in Buchi sheep of Cholistan desert.” FapUNIFESP (SciELO) 78. http://dx.doi.org/10.1590/1678-4162-13631
2. Abhishek Raj, Faiz Ali Khan, Hizbullah Khan, Khawar Ali Shahzad, Xin-Ying Ji, Shekhar Singh. 2026. “Soybean oil emulsion phytosterols promote ROS accumulation and apoptosis-associated signaling in human liver cells.” Elsevier BV 104. https://doi.org/10.1016/j.tice.2026.103828
3. Khawar Ali Shahzad, Wang Zhao, Cai Boyu, Li Xuran, Lv Xiaohui, Wang Yanhong, Tan Fei. 2025. “Novel pharmaco-exosomal immunotherapy for united airway diseases: PLGA-encapsulated.” Stem Cell Research & Therapy 16. https://doi.org/10.1186/s13287-025-04624-8
4. Mushtaq Hussain Lashari, Anna Abdullah, Fouzia Nazir, Asma Iqbal, Muhammad Abrar Afzal, Umer Farooq, Musadiq Idris, Musarrat Abbas Khan, Faisal Shahzad, Mahad Azam, Khawar Ali Shahzad, Dalia Fouad, Farid Shokry Ataya, Shagufta Nasreen. 2025. “A preliminary study on digital quantification of ocular attributes in cattle as potential non-invasive indicators of anemia.” BMC Veterinary Research 21. https://doi.org/10.1186/s12917-025-04618-x
5. Imran Ibrahim Shaikh, Shekhar Singh, Yuling Feng, Khawar Ali Shahzad, Jianfeng Wang, Quan Zhou, Shuanghu Wang, Chunlai Zeng, Chuxiao Shao. 2025. “Therapeutic Targeting of Signaling Pathways in Abdominal Aortic Aneurysm: From Pathogenesis to Precision Medicine.” Drug Design, Development and Therapy 19. https://doi.org/10.2147/DDDT.S563581
6. Zhao Wang#, Khawar Ali Shahzad#, Ding Li, Boyu Cai, Zilin Huang, Maoxiang Xu, Jiaojiao Li, Dong Chen, Fei Tan. 2025. “PLGA-loaded monascin intranasal delivery system: sustained-release and immunomodulatory effect for treatment of allergic rhinitis by improving regulatory T cells function.” ACS Applied Bio Materials 21. doi: 10.1021/acsabm.5c00387
7. Threem Zia, Irfana Liaqat, Khawar Ali Shahzad, Mushtaq Hussain Lashari, Dalia Fouad, Farid S. Ataya, Saman Alam, Areeba Saeed. 2025. “Ameliorative effect of Fagonia indica-coated chitosan nanoparticles on the ovulatory pattern in PCOS rat model.” Journal of Ovarian Research 18. https://doi.org/10.1186/s13048-025-01635-0
8. Khawar Ali Shahzad, Zhao Wang, Xuran Li, Jiaojiao Li, Maoxiang Xu, Fei Tan. 2024. “Immunomodulatory effect of PLGA-encapsulated mesenchymal stem cells-derived exosomes for the treatment of allergic rhinitis.” Frontiers in Immunology 15. 10.3389/fimmu.2024.1429442
9. Jiaojiao Li, Xuran Li, Xiangyu Li, Zhanping Liang, Zhao Wang, Khawar Ali Shahzad, Maoxiang Xu, Fei Tan. 2024. “Local Delivery of Dual Stem Cell-Derived Exosomes Using an Electrospun Nanofibrous Platform for the Treatment of Traumatic Brain Injury.” ACS Applied Materials & Interfaces 16. 10.1021/acsami.4c05004
10. Ding Li, Danni Li, Zhao Wang, Jiaojiao Li, Khawar Ali Shahzad, Yanhong Wang, Fei Tan. 2024. “Signaling pathways activated and regulated by stem cell-derived exosome therapy.” Cell & Bioscience 14. https://doi.org/10.1186/s13578-024-01277-7
11. Fei Tan, Xuran Li, Xiao Li, Maoxiang Xu, Khawar Ali Shahzad, Lei Hou. 2024. “GelMA/PEDOT:PSS Composite Conductive Hydrogel-Based Generation and Protection of Cochlear Hair Cells through Multiple Signaling Pathways.” Biomolecules 14. https://doi.org/10.3390/biom14010095
12. Zhao Wang#, Khawar Ali Shahzad#, Xuran Li, Boyu Cai, Maoxiang Xu, Jiaojiao Li, Fei Tan. 2024. “lmmunomodulatory effect of mesenchymal stem cells-derived extracellular vesicles to modulate the regulatory T cells and Th1/Th2 imbalance in peripheral blood mononuclear cells of patients with allergic rhinitis. .” Scandinavian Journal of lmmunology 0. DOI: 10.1111/sji.13416
13. Muhammad Younis, Sana Shaikh, Khawar Ali Shahzad, Fei Tan, Zhao Wang, Mushtaq Hussain Lashari. 2024. “Amrubicin encapsulated PLGA NPs inhibits the PI3K/AKT signaling pathway by activating PTEN and inducing apoptosis in TMZ-resistant Glioma.” Biomedical Materials 19. https://doi.org/10.1088/1748-605X/ad1bb2
14. Haq Nawaz Abbasi, Waqar Ahmad, Khawar Ali Shahzad*, Xiwu Lu. 2024. “Evaluating the potential of Abelmoschus esculentus, Solanum melongena, and Capsicum annuum Spp. for nutrient and microbial reduction from wastewater in hybrid constructed wetland.” Environmental Monitoring and Assessment 22. 10.1007/s10661-024-12474-9
15. Fei Tan, Xuran Li, Zhao Wang, Jiaojiao Li, Khawar Shahzad, Jialin Zhen. 2024. “Clinical applications of stem cell-derived exosomes.” Signal Transduction and Targeted Therapy 9. https://doi.org/10.1038/s41392-023-01704-0
16. Imran Ibrahim Shaikh, Ramesh Bhandari, Shekhar Singh, Khawar Ali Shahzad, Xu Zhu, Chuxiao Shao, Liming Cheng. 2024. “Therapeutic potential of EVs loaded with CB2 Receptor Agonist in Spinal Cord Injury via the Nrf2/HO-1 pathway.” Redox Report 29. DOI: 10.1080/13510002.2024.2420572
17. Mavra Saleem†, Khawar Ali Shahzad†, Munazzah Marryum, Shekhar Singh, Quan Zhou, Siting Du, Shuanghu Wang, Chuxiao Shao, Imran Ibrahim Shaikh. 2024. “Exosome-based therapies for inflammatory disorders: a review of recent advances.” Stem Cell Research & Therapy 15. https://doi.org/10.1186/s13287-024-04107-2
18. Fei Tan, Maoxiang Xu, Xuran Li, Zhao Wang, Jiaojiao Li, Khawar Ali Shahzad. 2024. “Biomaterial-Facilitated Local Delivery of Stem Cell-Derived Small Extracellular Vesicles: Perspectives in Surgical Therapy.” Advanced Therapeutics 2300387. 10.1002/adtp.202300387
19. Mubashir Hussain, Xu He, Mingyue Chen Xinye Ni, Yichuan Wang, Yan Deng, Zhu Chen, Song Li, Hui Chen, Khawar Ali Shahzad, Jianqing Li, Yongjun Tang, Xiuping Zhou, Bin Liu. 2023. “Optical Spectroscopy Based Microfluidic Platform for Detecting Pathogens Using Immunomagnetic Separation.” Journal of Nanoelectronics and Optoelectronics 18. 10.1166/jno.2023.3523
20. Yan Li, Zhongjie Qin, Lin Zhou, Khawar Ali Shahzad*, Delin Xia. 2021. “Collective influence of substrate chemistry with physiological fluid shear stress on human umbilical vein endothelial cells.” Cell Biology International 45. 10.1002/cbin.11632
21. Khawar Ali Shahzad, Zhongjie Qin, Yan Li, Delin Xia. 2020. “Focal Adhesion and Cytoskeleton Systems are Mechanotransductor and Mechanoreceptors in Fluid Shear Stress-Induced Endothelial Cells Response.” Biocell 44. 10.32604/biocell.2020.08500
22. Khawar Ali Shahzad, Muhammad Naeem, Lei Zhang, Xin Wan, Weiya Pei, Song Shilong, Chuanlai Shen. 2019. “Design and Optimization of PLGA Particles to Deliver Immunomodulatory Drugs for the Prevention of Skin Allograft Rejection.” Immunological Investigations 6. 10.1080/08820139.2019.1695134
23. Lei Zhang, Shilong Song, Xiaoxiao Jin, Xin Wan, Khawar Ali Shahzad, Weiya Pei, Chen Zhao, Chuanlai Shen. 2019. “An artificial antigen-presenting cell delivering 11 immune molecules expands tumor antigen-specific CTLs in ex vivo and in vivo murine melanoma models.” Cancer Immunology Research 7. 10.1158/2326-6066.CIR-18-0881
24. Weiya Pei, Xin Wan, Khawar Ali Shahzad, Lei Zhang, Shilong Song, Xiaoxiao Jin, Limin Wan, Chen Zhao, Chuanlai Shen. 2018. “Direct modulation of myelin-autoreactive CD4+ and CD8+ T cells in EAE mice by a tolerogenic nanoparticle co-carrying myelin peptide-loaded major histocompatibility complexes, CD47 and multiple regulatory molecules.” International journal of nanomedicine 13. 10.2147/IJN.S164500
25. Wan Xin, Pei Weiya, Zhang Yiming, Zhang Lai, Khawar Ail Shahzad, Xu Tao, Shen Chuanlai. 2018. “Inconsistence between number and function of autoreactive T cells in the course of experimental autoimmune encephalomyelitis.” Immunological Investigations 47. 10.1080/08820139.2017.1367008
26. Khawar Ali Shahzad, Xin Wan, Lei Zhang, Weiya Pei, Aifeng Zhang, Muhammad Younis, Wei Wang, Jin Xiaoxiao, Zhao Chen, Song Shilong, Chuanlai Shen. 2018. “On-target and direct modulation of alloreactive T cells by a nanoparticle carrying MHC alloantigen, regulatory molecules and CD47 in a murine model of alloskin transplantation.” Drug Delivery 25. 10.1080/10717544.2018.1447049
27. Xin Wan, Weiya Pei, Khawar Ali Shahzad, Lei Zhang, Shilong Song, Xiaoxiao Jin, Limin Wang, Chen Zhao, Chuanlai Shen. 2018. “A tolerogenic artificial antigen-presenting cell durably ameliorates EAE by directly and selectively modulating myelin peptide-autoreactive CD4+ and CD8+ T cells.” Journal of Immunology 201. 10.4049/jimmunol.1800108
28. Lei Zhang, Limin Wang, Khawar Ali Shahzad, Tao Xu, Xin Wan, Weiya Pei, Chuanlai Shen. 2017. “Paracrine release of IL-2 and anti-CTLA-4 enhances the ability of artificial polymer antigen-presenting cells to expand antigen-specific T cells and inhibit tumor growth in a mouse model.” Cancer Immunology Immunotherapy 66. 10.1007/s00262-017-2016-9
29. Wei Wang†, Khawar Ali Shahzad†, Miaochen Li, Aifeng Zhang, Lei Zhang, Tao Xu, Xin Wan, Chuanlai Shen. 2017. “An Antigen-Presenting and Apoptosis-Inducing Polymer Microparticle Prolongs Alloskin Graft Survival by Selectively and Markedly Depleting Alloreactive CD8+ T Cells.” Frontiers in Immunology 8. 10.3389/fimmu.2017.00657
30. Chuanlai Shen, Tao Xu, You Wu, Xiaoe Li, Lingzhi Xia, Wei Wang, Khawar Ali Shahzad, Lei Zhang, Xin Wan, Jie Qiu. 2017. “Frequency and reactivity of antigen-specific T cells were concurrently measured through the combination of artificial antigen presenting cell, MACS and ELISPOT.” Scientific Reports 7. 10.1038/s41598-017-16549-1
31. Wei Wang, Kun Fang, Miao-Chen Li, Di Chang, Khawar Ali Shahzad, Tao Xu, Lei Zhang, Ning Gu, Chuan-Lai Shen. 2016. “A biodegradable killer microparticle to selectively deplete antigen-specific T cells in vitro and in vivo.” Oncotarget 12176. 10.18632/oncotarget.7519
32. Tao Xu, Xiaoe Li, You Wu, Khawar Ali Shahzad, Wei Wang, Lei Zhang, Chuanlai Shen. 2016. “A combination strategy for construction of peptide-?2m-H-2Kb single chain with overlap extension PCR and One-step cloning.” Journal of Microbiology and Biotechnology 26. 10.4014/jmb.1606.06038
33. Munir Muhammad Tanveer, Umar Sajid, Shahzad Khawar Ali, Shah Muhammad Ali. 2016. “Potential of Magnetic Nanoparticles for Hepatitis B Virus Detection.” Journal of Nanoscience and Nanotechnology 16. https://doi.org/10.1166/jnn.2016.13759
34. Wei Wang, Kun Fang, Xiaobing Wang, Miaochen Li, You Wu, Feng Chen, Khawar Ali Shahzad, Ning Gu and Chuanlai Shen. 2015. “Antigen-specific killer polylactic-co-glycolic acid (PLGA) microspheres can prolong alloskin graft survival in a murine model.” Immunological Investigations 44. 10.3109/08820139.2015.1014098
35. TAHIR, M., SHAHZAD, K.A., MUHAMMAD, K., HABEEB, M.A., QAZI, M.U.H. AND SALEEM, M. 2013. “Reliability of urine pus cell counts on microscopy correlated with urine culture colony counts on culture media for UTI during pregnancy.” Pakistan Journal of Zoology 33. www.zsp.com.pk
Research and Development Projects
1. The in vitro appropriate substrate chemistry technique to optimally combine the physiological fluid shear stress in the case of bone and skin tissue engineering
With: Innovative research team of Taizhou Polytechnic College, Taizhou, Jiangsu, China (2018-06 - 2020-02)
Innovative research team of Taizhou Polytechnic College
The Islamia University of Bahawalpur was established in the year 1975 by upgrading historical institute of the Jamia Abbasia.
